Crystallization of Aspergillus japonicus lipase by hanging drop method
نویسنده
چکیده
Lipase purified from Aspergillus japonicus isolated from Ropalidia marginata paper nest was attempted for crystallization by hanging drop method. 2-methyl2, 4-pentanediol (MPD) was used as the precipitating agent and single crystals of A. japonicus lipase were obtained by using protein concentrated to 10 mg/mL and a reservoir solution containing 10-20% MPD buffered with 150 mM cacodylate buffer (pH, 6.8). Small crystals of 0.05 x 0.05 x 0.03 mm dimension resulted at the end of a week.
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Approach for growth of high-quality and large protein crystals
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